Glossary & References / Keywords List / Definitions:

Term to be defined Definition Synonyms & Keywords
Indirect fluorescent antibody (IFA) assay - "A serological test used to detect agent-specific antibodies. Both infected and uninfected cells are attached to a microscope slide. Test serum, diluted, is placed over the cells and left to allow antibody binding to antigens; unbound antibodies are then washed off the slide. A fluorescent-labelled species-specific anti-immunoglobulin antibody is added to the slide and allowed to bind to the antibodies; unbound labelled antibody is washed off then a fluorescent microscope is used to observe specific fluorescence. These assays are rapid, inexpensive and highly sensitive, but their interpretation is subjective and requires a skilled observer." (J495.51.w2)
  • IFA
  • IFAT (indirect fluorescent antibody test)